t-C圜IF requires no specialized instruments or reagents and is compatible with super-resolution imaging we demonstrate its application to quantifying signal transduction cascades, tumor antigens and immune markers in diverse tissues and tumors. t-C圜IF generates up to 60-plex images using an iterative process (a cycle) in which conventional low-plex fluorescence images are repeatedly collected from the same sample and then assembled into a high-dimensional representation. We describe a tissue-based cyclic immunofluorescence (t-C圜IF) method for highly multiplexed immuno-fluorescence imaging of formalin-fixed, paraffin-embedded (FFPE) specimens mounted on glass slides, the most widely used specimens for histopathological diagnosis of cancer and other diseases.
The architecture of normal and diseased tissues strongly influences the development and progression of disease as well as responsiveness and resistance to therapy.
